Biol. Pharm. Bull. 30(10) 1943—1946 (2007)
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چکیده
trescine are major polyamines in mammalian cells, and they play important roles in cell growth and differentiation. Although a variety of analytical methods for polyamines have been developed, there is still a need for a simpler, more sensitive and accurate method. The mass spectrometric method can simultaneously separate and identify polyamines on the basis of the difference of their mass without use of column. A quadrupole mass spectrometer with ionspray ionization interface (ISI-Q MS) is the first one used to determine polyamine concentrations in rat tissues. Recent progress in mass spectrometer has made it possible to use a time-of-flight mass spectrometer with electrospray ionization interface (ESI-TOF MS), which is more sensitive and provides a higher resolution. The present paper describes a greatly improved ESI-TOF MS method to determine the concentrations of spd and spm in mammalian cells. The data showed that this method had a high sensitivity and reproducibility, and allowed the measurement of multiple samples.
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The reduction of methaemoglobin in red blood cells and studies on the cause of idiopathic methaemoglobinaemia.
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